Table 1.

Summary of Simulations

Protein	CG or AT	Name	Lipid Compositiona	Replicates × Duration
GCGRapo	CG	Binary mixture	POPC, Chol	10 × 10 μs
			3:1
GCGRapo	CG	Mixed lipid: 0% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, CHOL	10 × 10 μs
			25:25:5:5:15:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRapo	CG	Mixed lipid: 5% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, GM3b, CHOL	10 × 10 μs
			22.5:22.5:5:5:15:5:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRapo	CG	Mixed lipid: 10% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, GM3b, CHOL	10 × 10 μs
			20:20:5:5:15:10:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRapo	CG	Mixed lipid: 10% GM1	EC – POPC, DOPC, POPE, DOPE, Sphb, GM1b, CHOL	3 × 10 μs
			20:20:5:5:15:10:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRapo	CG	Mixed lipid: 15% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, GM3b, CHOL	5 × 10 μs
			17.5:17.5:5:5:15:15:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRapo	CG	Mixed lipid: 20% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, GM3b, CHOL	5 × 10 μs
			15:15:5:5:15:20:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRpept	CG	Mixed lipid: 10% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, GM3b, CHOL	10 × 10 μs
			20:20:5:5:15:10:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRΔ_pept	CG	Mixed lipid: 10% GM3	EC – POPC, DOPC, POPE, DOPE, Sphb, GM3b, CHOL	10 × 10 μs
			20:20:5:5:15:10:25
			IC – POPC, DOPC, POPE, DOPE, POPS, DOPS, PIP2, CHOL
			5:5:20:20:8:7:10:25
GCGRapo	AT	Mixed lipid: 10% GM3	EC – POPC, (18:1/16:0) GM3, CHOL	2 × 0.5 μs
			65:10:25
			IC – POPC, PIP2, CHOL
			65:10:25

EC, extracellular leaflet; IC, intracellular; Sph, sphingomyelin.

^aPIP₂ was modeled with 1-palmitoyl-2-oleoyl tails.

^bLipids with C(d18:1/18:0) N-stearoyl-D-erythro tails.