Table 1.
Comparison of NS1 detection among RT-PCR, ELISA, lateral flow and SPR.
Sample number | Test sample | RT-PCR | ELISA |
Lateral flow | SPR |
||||
---|---|---|---|---|---|---|---|---|---|
IgM | IgG | NS1 | Conc. (nM) | Signal (degrees) | Result | ||||
1 | Positive | + | + | − | + | + | 3.134 | 0.00252 | + |
2 | Positive | + | − | + | + | + | 0.333 | 0.00125 | + |
3 | Positive | + | − | + | + | + | 0.313 | 0.00115 | + |
4 | Positive | + | − | − | + | + | 0.301 | 0.00113 | + |
5 | Positive | + | − | + | + | + | 3.061 | 0.00234 | + |
6 | Positive | + | + | + | + | − | 0.884 | 0.00173 | + |
7 | Positive | + | − | + | + | + | 2.312 | 0.00229 | + |
8 | Positive | + | − | + | + | + | 5.182 | 0.00314 | + |
9 | Positive | + | − | + | + | + | 4.353 | 0.00297 | + |
10 | Positive | + | − | + | + | + | 4.352 | 0.00273 | + |
11 | Positive | + | + | + | + | + | 3.381 | 0.00264 | + |
12 | Positive | + | + | + | + | + | 7.124 | 0.00398 | + |
13 | Positive | + | + | + | + | + | 2.217 | 0.00194 | + |
14 | Positive | + | − | + | + | + | 0.543 | 0.00136 | + |
15 | Positive | + | + | − | + | + | 1.823 | 0.00188 | + |
16 | Positive | + | − | + | + | + | 0.342 | 0.00127 | + |
17 | Normal | − | − | − | − | − | 0.005 | 0.00042 | − |
18 | Normal | − | − | − | − | − | 0.034 | 0.00075 | − |
19 | Normal | − | − | + | − | − | 0.005 | 0.00044 | − |
20 | Normal | − | − | + | − | − | 0.027 | 0.00074 | − |
21 | Normal | − | − | − | − | − | 0.004 | 0.00040 | − |
22 | Normal | − | − | + | − | − | 0.006 | 0.00047 | − |
23 | Normal | − | − | − | − | − | 0.003 | 0.00033 | − |
24 | Normal | − | − | + | − | − | 0.007 | 0.00059 | − |
25 | Normal | − | − | + | − | − | 0.007 | 0.00057 | − |
26 | Normal | − | − | + | − | − | 0.003 | 0.00034 | − |