Table 1.
Expression of apoptosis and DNA damage markers.
| Sham - I | tIRI - I | tIRI + Apocynin - I1 | Sham - C | tIRI - C | tIRI + Apocynin - C1 | |
|---|---|---|---|---|---|---|
| Caspase 9 (A405) | 0.03 ± 0.00 | 0.05 ± 0.01* | 0.03 ± 0.006# | 0.03 ± 0.01 | 0.03 ± 0.004 | 0.03 ± 0.01 |
| Caspase 3 (mmol pNA/min/ml) | 15 ± 4.5 | 33 ± 7.1* | 16 ± 5.4# | 16 ± 6.4 | 16 ± 3.8 | 13 ± 2.4 |
| Survivin (pg/ml) | 968 ± 31 | 591 ± 91* | 1079 ± 133# | 898 ± 116 | 1128 ± 121 | 1035 ± 249 |
| Birc5 (Normalized fold expression) | 1.0 ± 0.0 | 0.39 ± 0.07* | 0.90 ± 0.17# | 1.0 ± 0.0 | 1.1 ± 0.09 | 0.90 ± 0.17 |
| p-ASK1 (IF intensity) | 225 ± 15 | 1,028 ± 123 | 319 ± 62 | 357 ± 39 | 393 ± 49 | 392 ± 44 |
| p-JNK (IF intensity) | 280 ± 29 | 861 ± 125 | 198 ± 42 | 350 ± 26 | 301 ± 52 | 236 ± 24 |
| γ-H2AX (IF intensity) | 728 ± 56 | 1216 ± 112 | 793 ± 67 | 745 ± 85 | 784 ± 120 | 814 ± 97 |
Data are presented as mean ± SD (n = 6).
Data analysis was determined by the one-way analysis of variance (ANOVA) accompanied by the Holms-Sidak multiple comparisons test.
1
Rats received an i.p. injection of apocynin (50 mg/kg) 30 min prior to reperfusion.
*
tIRI compared to sham.
#
tIRI + Apocynin compared to tIRI.