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. 2020 Jul 22;10:12175. doi: 10.1038/s41598-020-68887-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hot regions in tertiary (a), secondary (b) and primary (c) structures of αS. Red, yellow and cyan colors are indicated the first, second and third priorities of hot regions, respectively. The gray color corresponds to the disorder part of αS. (d) Snapshots of αS intervals of 25 ns. The first, second and third priorities of hot sites are indicated red, yellow and cyan, respectively. The significant changes for each region are identified by circles in 25, 75 and 325 ns, respectively. The gray color corresponds to the disorder part of αS. Each configuration provided by VMD version 1.9.3²⁷.