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. 2020 Jun 23;119(2):389–401. doi: 10.1016/j.bpj.2020.06.013

Figure 3.

Figure 3

Electron paramagnetic resonance (EPR) spectroscopy of site-directed spin-labeled mouse melanopsin in DM. (A) Cartoon schematic of the EPR measurement of melanopsin. Affinity-purified melanopsin in dodecyl maltoside solution was site directed and spin labeled at C268 on the third cytoplasmic loop. (B) EPR spectrum of MTSL in solution with no protein. (C and D) EPR spectra of mouse melanopsin, MTSL-conjugated at C268. EPR spectra were recorded in darkness under dim red-light illumination at room temperature. (C) EPR spectrum of full-length melanopsin C268 in darkness (black trace) and after 30-s light illumination (red trace). (D) EPR spectrum of C-terminally truncated melanopsin C268 in darkness (black trace) and after 30-s light illumination (red trace).