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Phusion HF (Thermo Scientific) pipetting scheme.
| Volume per tube | |
|---|---|
| 5 × Phusion HF Buffer | 10 µl |
| 10 mM dNTPs | 1 µl |
| fwd Primer (10 pmol/µl) | 1 µl |
| rev Primer (10 pmol/µl) | 1 µl |
| Template DNA (0.1 µg/µl) | 1 µl |
| Dimethyl sulfoxide | 1.5 µl |
| Phusion HF DNA Polymerase | 0.5 µl |
| ad 50 µl ddH2O |
