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. 2020 Jul 22;10:12200. doi: 10.1038/s41598-020-68948-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in reactive oxygen species level in PBMCs (A) and HL-60 cells (B) pre-incubated with 40 µM CORM-2 and 40 µM iCORM-2 for 1 h at 37 °C and then incubated with 1 mM or 5 mM H₂O₂ at 37 °C. Changes in RFU were measured after 15 min, 30 min, 45 min and 60 min. Each value represents the mean ± SD, which was calculated from 6 individual experiments; *P < 0.05, ***P < 0.001 negative control or DMSO vs. H₂O₂ or CORM-2/iCORM-2, respectively; #P < 0.05, ##P < 0.01, ###P < 0.001 H₂O₂ vs. CORM-2/iCORM-2 + H₂O₂; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 CORM-2 + H₂O₂ vs. iCORM-2 + H₂O₂.