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. 2020 Jul 22;10:12127. doi: 10.1038/s41598-020-68847-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) (i–iii) Change of DPBF absorption spectra after photo-irradiation of the photosensitizers. Methylene blue (i), Pyro (ii) or azo-PDT (iii) was dissolved at 1 μM in EtOH. DPBF was added to the solution to a final concentration of 40 μM. The solution was irradiated with light filtered around the maximum absorption wavelength (670 nm). The absorption spectrum was obtained every 10 s. (iv) Decay curves of the 410 nm absorption bands in i, ii, and iii. The power of LED lamp was 150 W and the distance of irradiation was 80 cm; (b) Absorption (i) and fluorescent (ii) spectra of 5 µM of SiR-665, azo-PDT and Pyro in PBS.