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. 2020 Jul 22;10:12127. doi: 10.1038/s41598-020-68847-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Time-dependent changes in the fluorescence intensity of azo-PDT (5 μM) in the presence of mouse liver microsomes (226 μg/3 mL); (b) fluorescent responses of azo-PDT (5 μM) towards various analytes. Data shown were the fluorescence intensity of the probe at 680 nm after being incubated with various analytes for 60 min. All fluorescence data were collected in PBS (pH 7.4, 100 mM) containing 5% DMF as co-solvent at 37 °C with λ_ex 420 nm.