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. 2020 Jul 22;10:12139. doi: 10.1038/s41598-020-69085-w

Figure 2.

Figure 2

Steatohepatitis in mice leads to Klf10 loss of rhythmicity in the liver. WT male mice were fed on a control diet (CD) (n = 3–4 mice per ZT) or a methionine and choline deficient diet (MCDD) (n = 5–6 mice per ZT) for 4 weeks and the blood and liver were sampled around the clock every 6 h. (A) Gene expression of Bmal1, Rev-erbα, Per2, Cry1, Rev-erb-β, Rorc and Dbp in mice fed a CD or a MCDD. (B) Gene expression of Klf10, Klf11, Klf4 and Klf6. All data are expressed as mean ± SEM. Gene expressions are normalized to B2m and expressed relative to the CD ZT3 level. Rhythmicity of the hepatic gene expressions was evaluated by nonlinear regression cosine fitting analysis (cosinor) (Supplementary Table S2). The rhythmicity of each parameter is indicated on their respective graph with the corresponding color. ZT3 values were double-plotted to complete the 24 h cycle. *, rhythmic (p < 0.05), nsr, non-significantly rhythmic, nd, not detected.