Table 1.

The ancestors and derived lines from the current study are listed

V. fischeri EM17 and EM17Tn7 are isogenic strains that are sensitive (CAMS) and resistant (CAMR) to the antibiotic chloramphenicol, respectively. Each was evolved for 2000 generations to the same pH selection regimes, FLM pH 6.0, 7.4, 8.0, 10.0, and 6.0/10.0 (n=20 each). Hence, two ancestral varieties (EM and TN) underwent 2000 generations of evolution in each pH selection regime, which led to two varieties of derived lines (EM2000 and TN2000).

FLM pH Lineage	ET (CAMS) and TN (CAMR) Varieties	Nickname
Ancestral lines	EM and TN -unevolved line	Ancestors
FLM pH 6.0 derived lines	EM2000 pH 6.0 and TN2000 pH 6.0 evolved line lower pH limit	Acid specialists
FLM pH 7.4 derived lines	EM2000 pH 7.4 and TN2000 pH 7.4 evolved line optimal pH control	Optimists
FLM pH 8.0 derived lines	EM2000 pH 8.0 and TN2000 pH 8.0 evolved line middle pH control	Centrists
FLM pH 10.0 derived lines	EM2000 pH 10.0 and TN2000 pH 10.0 evolved line upper pH limit	Alkaline specialists
FLM pH 6.0/10.0 derived lines	EM2000 pH 6.0/10.0 and TN2000 pH 6.0/10.0 evolved line fluctuating lower upper	pH generalists