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. 2020 Apr 28;166(6):579–592. doi: 10.1099/mic.0.000916

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© 2020 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 5. — Effect of qspB overexpression on sporulation and colony size. (a) The ability to sporulate in CBMS was assessed for the C. acetobutylicum ATCC 824 parent strain containing the empty pMTL85143 vector (black bars) and the overexpression plasmid pMTL85143-qspB (white bars). Data represent the mean of four independent cultures with error bars indicating the standard deviation. Significant differences (P≤0.05) compared to the vector controls are indicated by an asterisk next to the relevant measurement. (b) 5-day-old colonies of C. acetobutylicum carrying the empty pMTL85143 vector (left) and overexpression plasmid pMTL85143-qspB, respectively, on CGM agar.