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. 2020 Jul 23;51:93. doi: 10.1186/s13567-020-00818-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Anti-fimbriae antibodies from the immunized groups inhibited adherence of porcine ETEC strains to porcine small intestinal cell lines. A Serum samples from the immunized groups significantly inhibited K88+, F18+, K99+, 987P+, and F41+ ETEC strains to the IPEC-1 cell line. B Serum samples from the immunized groups significantly inhibited K88+, F18+, K99+, 987P+, and F41+ ETEC strains to the IPEC-J2 cell line. The number of adherent bacteria (CFU) was converted to percentages, with CFU from cells treated with control group serum set to 100%. The results are expressed as mean ± standard deviation of at least three independent experiments in three wells. **Indicates statistically significant difference when compared to the ETEC strains treated with control serum (P < 0.01).