Table 1.
Strains and plasmids used in this study.
| Strains/plasmids | Relevant properties | Source |
|---|---|---|
| BL21 | F−ompT hsdS(r−Bm−B) gal dcm | Novagen |
| C83901 | wild-type O8: H19:K88ac LT/STa/STb | CIVDC |
| 2134P | wild-type O157:H19 F18ac/4p-/STa/STb | Casey et al. [31] |
| 1592 | Wild-type 987P+ ETEC | CIVDC |
| 1593 | Wild-type K99+ ETEC, STa | CIVDC |
| 1594 | Wild-type F41+ ETEC | CIVDC |
| 1901 | FaeG–F41–FanC–FasA MEFA recombinant strain | This study |
| 1902 | FedF–FasA–F41–FanC MEFA recombinant strain | This study |
| 1903 | FaeG subunit (K88 fimbriae) recombinant strain | This study |
| 1904 | FanC subunit (K99 fimbriae) recombinant strain | This study |
| 1905 | FasA subunit (987P fimbriae) recombinant strain | This study |
| 1906 | FedF subunit (F18 fimbriae) recombinant strain | This study |
| 1907 | Fim41a subunit (F41 fimbriae) recombinant strain | This study |
| Plasmid | ||
| pET28α+ | Novagen | |
| p1908 | FaeG–F41–FanC–FasA MEFA in pET28α+ | This study |
| p1909 | FedF–FasA–F41–FanC MEFA in pET28α+ | This study |
| p1910 | FaeG–FedF–F41–FanC–FasA MEFA in pET28α+ | This study |