Table 2.
Primer | Sequences (5′-3′) |
---|---|
MEFA-F1 | CGCGGATCCATGAAAAAAACCCTGATCGCTCTGGC |
MEFA-F2 | ATCACCTACTACGGACCCGGACCTGGTCGTCTGAAATAC |
MEFA-R1 | GAGCGTCGACTTACTGGATTTCGAA AACGATCGGAAC |
MEFA-R2 | GTATTTCAGACGACCAGGTCCGGGTCCGTAGTAGGTGAT |
FasA-F | CGCGGATCCATGAGAATGAAAAAATCCGC |
FasA-R | CAGCGTCGACTTACGGTGTACCTGCTGAA |
FanC-F | CGCGGATCCATGAAAAAAACACTGCTAGC |
FanC-R | CAGCGTCGACTTACATATAAGTGACTAAG |
FedF-F | CTAGCTAGCATGCGTTTAAAATATATCTTG |
FedF-R | CAGCGTCGACTTACTGTATCTCGAAAAC |
FaeG-F | ATTCGGGATCCATGAAA AAGAC |
FaeG-R | CAGCGTCGACTTAGTAATAAGT |
Fim41a-F | CGCGGATCCATGAAAAAGACTCTGATTGC |
Fim41a-R | AACGAGCTCTTAACTATAAATAACGGTG |
Restriction sites, BamHI or NheI in forward primers, and SalI or SacI in reverse primers, were underlined.