Table 1.
Details of the ten candidate reference genes and primers used in the qPCR.
| Gene | Description | Primer: forward/reverse(5′-3′) | Length (bp) | Accession number |
|---|---|---|---|---|
| ACTB | β-Actin | F: AAGGCCAACCGCGAGAAGAT R: GCCAGAGGCGTACAGGGATA |
102 | NM_001101 |
| B2M | Beta-2 microglobulin | F: GTTTACTCACGTCATCCAGC R:AGACAAGTCTGAATGCTCCA |
141 | NM_004048 |
| GAPDH | Glyceraldehade-3-phosphate dehydrogenase | F: GCCTCCTGCACCACCAACTG R: CCATCACGCCACAGTTTCCC |
149 | NM_002046 |
| TUBB2a | Tubulin beta 2a | F: AACGCCACCCTCTCTGTCCA R: GCCGACACCAGGTGGTTGAG |
143 | NM_001069 |
| HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | F: ACTGAACGTCTTGCTCGAGA R: TGATGTAATCCAGCAGGTCA |
112 | NM_000194 |
| SDHA | Succinate dehydrogenase complex flavoprotein subunit A | F: AAAGATCACGTCTACCTGCA R: CATGTTATAATGCACGGTGG |
150 | NM_004168 |
| TBP | TATA-box binding protein | F: GTTCAGCAGTCAACGTCCCA R: TCATGGGGGAGGGATACAGT |
127 | NM_003194 |
| YWHAZ | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta | F: CAGGCTGAGCGATATGATGA R: CCTACGGGCTCCTACAACAT |
126 | NM_003406 |
| CYC1 | Cytochrome c isoform 1 | F: CCAAAACCATACCCCAACAG R: AGTCCTCACCACCATGCCTA |
103 | NM_001916 |
| GUSB | Glucuronidase beta | F: GTTCCTTTTGCGAGAGAGAT R: ACACGCAGGTGGTATCAGTC |
124 | NM_000181 |