Table 2.

Expression stability values of ten candidate reference genes in HepG2 analyzed by NormFinder.

Rank	WT	EtOH 100 mM	EtOH 200 mM	EtOH 400 mM	H2O2 200 μM	H2O2 400 μM	H2O2 800 μM	APAP 2.5 mM	APAP 5 mM	APAP 10 mM	CCl4 0.1%	CCl4 0.2%	CCl4 0.4%
1	HPRT1 0.011	HPRT1 0.004	CYC1 0.007	HPRT1 0.008	B2M 0.018	CYC1 0.013	TBP 0.011	HPRT1 0.013	GUSB 0.004	HPRT1 0.012	TBP 0.015	TBP 0.011	HPRT1 0.005
2	TUBB2a 0.012	TBP 0.005	TBP 0.009	TUBB2a 0.013	TUBB2a 0.019	TUBB2a 0.017	GAPDH 0.011	GUSB 0.015	CYC1 0.010	B2M 0.018	CYC1 0.017	ACTB 0.013	CYC1 0.008
3	CYC1 0.013	TUBB2a 0.005	SDHA 0.011	B2M 0.014	CYC1 0.020	TBP 0.019	SDHA 0.012	SDHA 0.018	TUBB2a 0.014	SDHA 0.019	SDHA 0.020	B2M 0.021	GUSB 0.009
4	B2M 0.017	YWHAZ 0.012	B2M 0.011	TBP 0.016	TBP 0.027	HPRT1 0.024	HPRT1 0.015	YWHAZ 0.022	ACTB 0.020	TUBB2a 0.020	HPRT1 0.021	TUBB2a 0.021	TUBB2a 0.016
5	TBP 0.018	SDHA 0.013	HPRT1 0.013	SDHA 0.018	GUSB 0.030	GUSB 0.025	B2M 0.017	CYC1 0.023	HPRT1 0.021	CYC1 0.022	TUBB2a 0.024	CYC1 0.021	SDHA 0.019
6	SDHA 0.019	ACTB 0.014	GAPDH 0.015	CYC1 0.020	SDHA 0.030	YWHAZ 0.027	TUBB2a 0.017	TBP 0.024	TBP 0.022	ACTB 0.024	GUSB 0.024	GUSB 0.022	TBP 0.024
7	ACTB 0.020	CYC1 0.022	GUSB 0.015	ACTB 0.025	YWHAZ 0.037	ACTB 0.031	CYC1 0.017	ACTB 0.028	YWHAZ 0.025	GUSB 0.025	YWHAZ 0.028	YWHAZ 0.022	B2M 0.029
8	GUSB 0.021	B2M 0.032	TUBB2a 0.016	GUSB 0.027	ACTB 0.039	B2M 0.037	ACTB 0.019	GAPDH 0.029	B2M 0.029	TBP 0.029	GAPDH 0.032	SDHA 0.024	GAPDH 0.031
9	YWHAZ 0.023	GAPDH 0.041	ACTB 0.020	YWHAZ 0.028	HPRT1 0.046	SDHA 0.043	GUSB 0.022	TUBB2a 0.030	SDHA 0.031	GAPDH 0.035	B2M 0.034	HPRT1 0.029	ACTB 0.037
10	GAPDH 0.029	GUSB 0.042	YWHAZ 0.023	GAPDH 0.041	GAPDH 0.061	GAPDH 0.048	YWHAZ 0.024	B2M 0.032	GAPDH 0.039	YWHAZ 0.037	ACTB 0.037	GAPDH 0.044	YWHAZ 0.039