Table 3.

Expression stability values of the reference genes calculated by BestKeeper in HepG2.

Rank	WT	EtOH 100 mM	EtOH 200 mM	EtOH 400 mM	H2O2 200 μM	H2O2 400 μM	H2O2 800 μM	APAP 2.5 mM	APAP 5 mM	APAP 10 mM	CCl4 0.1%	CCl4 0.2%	CCl4 0.4%
1	GUSB 1.57 ± 0.45	HPRT1 1.10 ± 0.24	SDHA 0.74 ± 0.20	B2M 1.57 ± 0.29	CYC1 1.37 ± 0.33	CYC1 1.49 ± 0.36	TBP 0.85 ± 0.24	SDHA 1.78 ± 0.44	CYC1 0.95 ± 0.25	GUSB 1.75 ± 0.59	GUSB 0.90 ± 0.26	HPRT1 1.67 ± 0.38	TBP 1.15 ± 0.30
2	ACTB 1.89 ± 0.39	CYC1 1.24 ± 0.3	TBP 1.00 ± 0.26	TBP 1.67 ± 0.44	TUBB2a 1.68 ± 0.48	YWHAZ 1.51 ± 0.35	CYC1 0.95 ± 0.24	TBP 2.50 ± 0.63	GUSB 1.47 ± 0.46	CYC1 2.60 ± 0.75	CYC1 1.10 ± 0.27	GUSB 1.68 ± 0.51	SDHA 1.53 ± 0.38
3	TUBB2a 2.22 ± 0.63	TBP 1.28 ± 0.33	CYC1 1.08 ± 0.27	SDHA 1.70 ± 0.46	SDHA 1.90 ± 0.51	GUSB 1.54 ± 0.47	B2M 1.08 ± 0.21	TUBB2a 2.83 ± 0.72	TUBB2a 1.59 ± 0.45	TUBB2a 3.25 ± 0.94	HPRT1 1.43 ± 0.32	TBP 1.72 ± 0.45	CYC1 1.72 ± 0.43
4	CYC1 2.41 ± 0.57	SDHA 1.79 ± 0.47	GUSB 1.12 ± 0.34	YWHAZ 2.08 ± 0.47	TBP 2.28 ± 0.61	HPRT1 1.77 ± 0.40	SDHA 1.16 ± 0.32	GUSB 2.97 ± 0.86	HPRT1 1.64 ± 0.41	ACTB 3.27 ± 0.77	B2M 1.99 ± 0.37	CYC1 1.84 ± 0.47	TUBB2a 1.97 ± 0.54
5	B2M 2.65 ± 0.48	TUBB2a 1.92 ± 0.54	ACTB 1.16 ± 0.25	HPRT1 2.12 ± 0.49	ACTB 2.58 ± 0.53	TUBB2a 1.80 ± 0.52	HPRT1 1.22 ± 0.29	YWHAZ 3.25 ± 0.71	B2M 1.74 ± 0.35	TBP 3.38 ± 0.98	TBP 2.19 ± 0.57	YWHAZ 2.40 ± 0.54	HPRT1 2.31 ± 0.53
6	TBP 2.78 ± 0.72	ACTB 2.01 ± 0.41	B2M 1.19 ± 0.22	CYC1 2.16 ± 0.53	B2M 2.84 ± 0.51	TBP 1.84 ± 0.49	GAPDH 1.39 ± 0.25	HPRT1 3.28 ± 0.76	YWHAZ 2.17 ± 0.53	B2M 3.90 ± 0.88	SDHA 2.42 ± 0.61	TUBB2a 2.58 ± 0.73	GUSB 2.72 ± 0.81
7	HPRT1 3.10 ± 0.70	YWHAZ 2.13 ± 0.47	TUBB2a 1.28 ± 0.37	TUBB2a 2.20 ± 0.62	GUSB 2.96 ± 0.88	ACTB 2.78 ± 0.57	GUSB 1.59 ± 0.50	CYC1 3.31 ± 0.82	ACTB 2.20 ± 0.50	HPRT1 4.04 ± 1.11	YWHAZ 2.45 ± 0.54	ACTB 2.84 ± 0.57	YWHAZ 2.97 ± 0.67
8	SDHA 3.41 ± 0.91	GUSB 2.18 ± 0.65	GAPDH 1.45 ± 0.25	GUSB 2.58 ± 0.78	YWHAZ 3.48 ± 0.78	B2M 3.14 ± 0.59	TUBB2a 1.62 ± 0.47	ACTB 3.39 ± 0.67	TBP 2.59 ± 0.72	SDHA 4.41 ± 1.30	TUBB2a 2.63 ± 0.74	B2M 2.92 ± 0.55	ACTB 3.92 ± 0.75
9	YWHAZ 3.63 ± 0.82	B2M 2.82 ± 0.51	HPRT1 1.55 ± 0.35	ACTB 3.37 ± 0.71	HPRT1 4.86 ± 1.10	SDHA 3.84 ± 1.05	ACTB 1.65 ± 0.36	B2M 4.86 ± 0.92	SDHA 2.68 ± 0.73	GAPDH 4.81 ± 0.97	ACTB 2.95 ± 0.60	SDHA 3.32 ± 0.84	B2M 4.41 ± 0.83
10	GAPDH 3.99 ± 0.66	GAPDH 3.30 ± 0.55	YWHAZ 2.32 ± 0.54	GAPDH 4.03 ± 0.71	GAPDH 5.26 ± 0.89	GAPDH 4.21 ± 0.71	YWHAZ 2.24 ± 0.53	GAPDH 4.95 ± 0.78	GAPDH 3.43 ± 0.63	YWHAZ 6.38 ± 1.64	GAPDH 3.42 ± 0.57	GAPDH 4.38 ± 0.73	GAPDH 4.46 ± 0.73