TABLE 2.
Linearity of qPCR assays
| Assay | Serial dilution sample |
Enterobacterales probe (Ent) |
Universal probe (Uni) |
||||
|---|---|---|---|---|---|---|---|
| Range (log10 CFU) | Slope | r 2 | Range (log10 CFU) | Slope | r 2 | ||
| A | K. pneumoniae | 3–9 | −3.441 | >0.99 | 3–9 | −3.374 | >0.99 |
| S. marcescens | 3–8 | −3.46 | >0.99 | 3–8 | −3.46 | >0.99 | |
| K. pneumoniae, E. coli, S. marcescens, Citrobacter freundii, and Enterobacter cloacae | 3–9 | −3.371 | >0.99 | 3–9 | −3.282 | >0.99 | |
| B | K. pneumoniae | 3–9 | −3.647 | >0.99 | 3–9 | −3.554 | >0.99 |
| S. marcescens | 4–8 | −3.594 | >0.99 | 4–8 | −3.683 | >0.99 | |
| K. pneumoniae, E. coli, S. marcescens, Citrobacter freundii, and Enterobacter cloacae | 3–9 | −3.529 | >0.99 | 3–9 | −3.33 | >0.99 | |