Table 1.

Hot spot app characteristics with the four configurable parameters: number of cells in the hot spot, heatmap and drawing radius (20× or 40× field of view), heatmap based on positive cells or on positive ratio, and hot spot shape (circle or contour heatmap)

Hot spot app	Tumor cell count	Radius	Positive/ratio	Shape
APP02	200	40×	Pos	Circle
APP03	1000	20×	Pos	Circle
APP04	200	20×	Pos	Circle
APP05	1000	20×	Ratio	Circle
APP06	200	20×	Ratio	Circle
APP08	200	40×	Ratio	Circle
APP09	1000	20×	Pos	Contour heatmap
APP10	200	20×	Pos	Contour heatmap
APP11	1000	40×	Pos	Contour heatmap
APP12	200	40×	Pos	Contour heatmap
APP13	1000	20×	Ratio	Contour heatmap
APP14	200	20×	Ratio	Contour heatmap
APP15	1000	40×	Ratio	Contour heatmap
APP16	200	40×	Ratio	Contour heatmap
APP20	400	40×	Ratio	Contour heatmap
APP21	600	40×	Ratio	Contour heatmap
APP22	800	40×	Ratio	Contour heatmap
APP23	1200	40×	Ratio	Contour heatmap
APP24	400	40×	Pos	Contour heatmap
APP25	600	40×	Pos	Contour heatmap
APP26	800	40×	Pos	Contour heatmap
APP27	1200	40×	Pos	Contour heatmap