TABLE 3.
Primer sequences used for qRT-PCR
| Protein description (abbreviation) | Protein accession no. | Primer sequence |
|---|---|---|
| 16S rRNA | ACCAGCGTCCTGTGCATGTC/AGTACGGCCGCAAGGCTAAAAC | |
| 3β-Hydroxy-Δ5-steroid dehydrogenase (3β-HSD) | V5XHS7 | TGGAACGGCTTTACCTGAA/TGTCGAACAGCTCGGTGTAGTA |
| Steroid C27-monooxygenase (Cyp125) | V5XGF9 | TGTCCTCCTTCGAGCTCATC/CACGAAGAAACCGAACTC |
| 3-Ketosteroid-Δ-1-dehydrogenase (KsdD) | V5XHH8 | ACTATGTCCGGCTCAACC/CCATACCGACCAGGTTCTT |
| NADH dehydrogenase subunit E (NuoE) | V5X9W2 | GATCTCCAGAATCGCGTCA/GTCGCCACGTTCTATTCG |
| NADH-quinone oxidoreductase (NuoG) | V5X8L5 | GCACCTACCGGACAGATCT/ACCCGTTCATCGAGTTGTTG |
| Pyruvate water dikinase | V5X8G4 | CGGTCGAAGAAGAACACG/AACAGACCAAGGCGAAGATG |
| Alanine dehydrogenase | V5XCR6 | AGCGTTTGACCCTGTCGTAG/CATCACCGACAACGACTTCA |