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eDNA was involved in the process of bacterial cell aggregation. (A) The level of eDNA release of the WT strain and spoVG-deletion strain. DNase I treatment was used as a negative control. (B) The cell clumps processed with DNase I treatment for 0, 2, 4, and 8 h were visualized by fluorescence microscopy. The fluorescent shuttle plasmid pALC was transformed into the WT, spoVG-deletion, and spoVG sasC double mutant strains. (Left) WT strain. (Right) spoVG-deletion strain.
