TABLE 3.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference no. |
|---|---|---|
| Strain | ||
| E. coli BL21(DE3) | F– ompT gal dcm lon hsdSB(rB− mB−) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Invitrogen |
| E. coli Rosetta(DE3) pLysS | F– ompT hsdSB(rB− mB−) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (Camr) | Novagen |
| Arthrobacter sp. 68b DSM 103156 | Soil isolate capable of degrading 2-methylpyridine, nicotine, phthalic acid, and pyridine | 25 |
| Plasmid | ||
| pET21b | T7 tag (N terminus), His tag (C terminus), Apr | Novagen |
| pET28b | T7 tag (N terminus), His tag (C and N termini), Kmr | Novagen |
| pETM11 | N-terminal His tag (MetLysHisHisHisHisHisHisProMetSerAspTyrAspIleProThrThrGluAsnLeuTyrPheGluGlyAla) was fused to the PyrB | 70 |
| pCDFduet | T7 tag (two), Smr | Novagen |
| pRSFduet | T7 tag (two), Kmr | Novagen |
| pACYCduet1 | T7 tag (two), Cmr | Novagen |