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. Author manuscript; available in PMC: 2021 Jul 2.
Published in final edited form as: Cell Stem Cell. 2020 Jul 2;27(1):35–49.e6. doi: 10.1016/j.stem.2020.06.004

Figure 5. Class-specific dysregulation of WNT signaling.

Figure 5.

(A) Schematic illustration of multiplex strategy to test autism mutations for WNT/βcatenin response during PFC growth. D35 Pooled PFC cultures are treated with the CHIR99021 (GSK3 inhibitor, 3μM) for 10 days and compared to untreated cultures. Stem cell proliferation is used as a read-out of WNT activity. (B) Correlation of PFC WNT response with PFC neurogenesis phenotype. 7/8 Class 1 mutations (blue edge) are hyporesponsive to WNT signaling (orange, FDR < 0.05). FDR calculated using Welch ANOVA with post-hoc comparisons to UMOD, p-values corrected for multiple comparisons using Benjamini, Krieger, Yekutieli method n = 4 differentiations from three MIX30 pools. See also Figure S5 and Table S3.