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. 2020 Jul 23;20:685. doi: 10.1186/s12885-020-07165-w

Fig. 2.

Fig. 2

PT induces apoptosis and cell cycle arrest. a HT-29 b SW480 c SW620 cells were treated with 5 and 10 μM of PT for 24 h. Total cell death including apoptosis and necrosis was analyzed by Annexin V/PI staining using flow cytometry. d HT-29 and e SW620 cell cycle distribution was measured by PI staining using flow cytometry and the percentage of cell population was determined in the G0/G1, S and G2/M phases. Results shown are representative of three independent experiment (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs control