Fig. 5.

PT blocks anti-apoptotic protein expression and activates cytochrome c and PARP cleavage. a HT-29 c SW480 e SW620 cells were exposed to different concentration of PT for 24 h at 37 °C. Soluble fraction of total cell lysates were immunoblotted with indicated antibodies. Cytosolic fraction of HT-29, SW480 and SW620 were prepared and immunoblotted for cytochrome c. b, d, f Density of the protein bands of three independent experiments were quantified and expressed as relative protein expression to actin. The bar graphs are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control. Full Length blots were presented in Supplementary Figure S1