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. 2020 Jul 23;18:115. doi: 10.1186/s12964-020-00584-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Phosphorylation of AMPK was suppressed by Kras mutation in CRC cells. a MTS analysis of DLD1 WT (+/−) and Kras mutation (G13D/−) cells treated with cetuximab (Cet) at the indicated doses for 48 h. b Apoptosis in DLD1 WT (+/−) and Kras mutation (G13D/−) cells treated with 5 nM Cet for 48 h was analyzed by nuclear staining with Hoechst 33258. c Apoptosis in DLD1 WT (+/−) and Kras mutation (G13D/−) cells treated with 5 nM Cet for 48 h was analyzed by Annexin V staining followed by flow cytometry. d Western blot of p-AMPK, AMPK, and Caspase-3 (C3) in the cells treated as in (b). e DLD1 WT (+/−) cells stably expressing control, Kras WT, or Kras mutant (G12V) by retrovirus transfection were treated with Cet at indicated doses for 48 h. The cell viability was analyzed by MTS assay. f DLD1 WT (+/−) cells stably expressing control, Kras WT, or Kras mutant (G12V) by retrovirus transfection were treated with 5 nM Cet for 48 h. The expression of indicated proteins was analyzed western blot. g MTs analysis of indicated cells treated with Cet at indicated doses for 48 h. The IC50 was calculated and plotted in the right panel. h The expression of p-AMPK, AMPK in the indicated cells treated with 5 nM Cet. Each experiment was repeated for 3 times. *, p < 0.05; **, p < 0.01