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. 2020 Jul 23;18:115. doi: 10.1186/s12964-020-00584-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Kras mutation suppressed the AMPK activation by glycolysis. a The cellular ATP level of DLD1 WT and Kras mutation cells was analyzed by luminescence assay. b The cellular ATP level in DLD1 WT (+/−) cells stably expressing control, Kras WT, or Kras mutant (G12V) by retrovirus transfection (c) The cellular ATP level in the indicated cell lines. d The cellular ATP level of HT29 and Difi cells cultured in media containing 20 mM glucose at indicated time. e HT29 and Difi cells cultured in the media with or without 20 mM glucose were treated with Cet (15 nM for HT29; 5 nM for Difi) for 48 h. The apoptosis was analyzed by Hoechst 33258 staining. f Western blot of indicated proteins in HT29 and Difi cells treated as in (e). g The cellular ATP level of DLD1 Kras mutated treated with 10 μM 3-BrPA for indicated time points. h The apoptosis of DLD1 Kras mutated cells treated with 10 μM 3-BrPA in combined with 5 nM Cet or 10 nM panitumumab (Pab). i Western blot of indicated proteins in DLD1 Kras mutated cells treated as in (h). Each experiment was repeated for 3 times. nd, p > 0.05; *, p < 0.05; **, p < 0.01