Fig. 3.

Cardiac arrest triggers the elevated level of molecules closely related to pyroptosis. a qRT-PCR results of the mRNA levels of NLRP3 and caspase-1 in sham-operated and post-cardiac arrest rats that were euthanized at 6, 12, 24, and 72 h after ROSC. b, c Western blotting results of NLRP3, pro-caspase-1, and cleaved caspase-1 in sham-operated and post-cardiac arrest rats that were euthanized at different time points after ROSC. dqRT-PCR results of the mRNA levels of GSDMD and caspase-11 expressed in hippocampus and cortex between the sham and post-surgery rats euthanized at 12 h after ROSC. e, f Western blotting results of GSDMD, cleaved GSDMD, pro-caspase-11, and cleaved caspase-11 in sham-operated and post-cardiac arrest rats. GSDMD-FL, full-length GSDMD. g, h Immunofluorescence results of NLRP3, ASC, and cleaved caspase-1 between the sham and cardiac arrest /cardiopulmonary resuscitation group, scale bar = 100 μm. All data in this figure are analyzed using parametric test. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham group. n = 5–6 per group. CA/CPR cardiac arrest and cardiopulmonary resuscitation