Table 1.
Represents antibodies used for immunoblotting analyses
| Antibody | Company | Molecular weight | Species | Dilution | Secondary antibody dilution | Notes |
|---|---|---|---|---|---|---|
| Actin | Millipore | 42 kDa | Mouse | 1/50,000 | 1/20000 | |
| APP | Millipore | ≈ 100 kDa | Mouse | 1/2000 | 1/5000 | antigen retrival (Michaud, Hallé et al. 2013) |
| Cox-2 | Santa Cruz | ≈ 75 kDa | Goat | 1/1000 | 1/5000 | |
| Iba-1 | Wako | 18 kDa | Rabbit | 1/1000 | 1/5000 | |
| ICAM | Santa Cruz | 85–110 kDa | Goat | 1/500 | 1/1000 | |
| LRP1 | CEDARLANE | 85 kDa | Rabbit | 1/1 000 | 1/40,000 | |
| MCP1 | Cell Signaling | 13 kDa | Rabbit | 1/1 000 | 1/5000 | |
| PSD95 | Neuromab | 95 kDa | Mouse | 1/2 000 | 1/20,000 | |
| Synaptophysin | Thermo Fisher Scientific | 34 kDa | Mouse | 1/10 000 | 1/100,000 | |
| Trem2 | R&D Systems | 40 kDa | Rabbit | 1/500 | 1/2000 | antigen retrival (Michaud, Hallé et al. 2013) |
| VCAM | Santa Cruz | 90–100 kDa | Rabbit | 1/1 000 | 1/10,000 | |
| NFkB p50 | CEDARLANE | 50 kDa | Rabbit | 1/1000 | 1/10,000 |
Table 1 represents antibodies used for immunoblotting analyses and all related information, including the name of the company, molecular weight, species, secondary antibodies, and dilution rates