Skip to main content
. 2019 Jun 5;146(11):dev174508. doi: 10.1242/dev.174508

Fig. 5.

Fig. 5.

V0-ATPase interacts genetically with GSLs and clathrin/chc-1 in intestinal polarity maintenance. (A) ERM-1::GFP-expressing control or let-767;sDp3 worms were silenced for control (L4440) or a mix of control (L4440), vha-1, chc-1 or aps-1 RNAi as indicated, and then imaged. (B-D) The quantification of the ERM-1::GFP basolateral/cytoplasmic ratio. Histograms are mean±s.e.m. on each panel, dots represent individual worms and the total number of worms from three (B-C) or four (D) independent experiments is indicated in brackets. ‘Additivity’ shows the sum of individual knockdowns or mutants. A specific statistical analysis was used to test the significance of the difference between double loss-of-function and additivity (see Materials and Methods). Arrows and arrowheads show the apical and basolateral PM, respectively. In all micrographs, worms are at the L4/young adult developmental stage. Scale bar: 5 µm. n.s. nonsignificant, *P<0.05, **P<0.01.