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. 2020 Jul 23;18:75. doi: 10.1186/s12958-020-00630-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Staining analysis of decellularized mouse ovary scaffolds. Decellularization was confirmed by two different staining methods; hematoxylin and eosin (H&E; a-d) and with the DNA-labelling dye DAPI (e-h). No dark-stained nuclei could be detected in the decellularized ovaries in any of the produced scaffolds (a-d). DAPI is considered to be a more than H&E and also confirmed the removal of DNA after decellularization (e-h). The green stain represents auto fluorescence from the ovarian tissue and was included in the images to enable the visualization of the extracellular matrix (ECM) structure