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. 2020 Jul 23;20:336. doi: 10.1186/s12935-020-01420-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — CDHI knockdown reversed the suppression action of circ-ITCH on ovarian cancer cell progression. a Western blot assay was applied to detect CDH1 protein level in A2780 and OVCAR3 cells transfected with pcDNA, circ-ITCH, circ-ITCH + si-con, or circ-ITCH + si-CDH1. b CCK-8 assay was performed to assess proliferation in transfected A2780 and OVCAR3 cells. c Transwell assay with Matrigel was used to measure invasion in A2780 and OVCAR3 cells. d, f Glucose, lactate and ATP assay kits were employed to examine glucose consumption, lactate production and ATP level in transfected A2780 and OVCAR3 cells, respectively. g Flow cytometry assay was conducted to assess apoptosis rate in transfected A2780 and OVCAR3 cells. **P < 0.01, ***P < 0.001