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. 2020 Jul 22;17:47. doi: 10.1186/s12987-020-00209-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Immunofluorescence analysis of isolated brain microvessels and capillaries (BMV) from the mouse (a–e) and human (f–i) brain. a CD31 immunofluorescence (red) in mouse BMV; b GSL-I lectin staining (green) in mouse BMV. c A composite image of CD31, GSL-I and Hoechst-stained nuclei (blue) of the same BMV. d Collagen IV immunofluorescence (green) in mouse BMV. e A composite image of collagen IV (green) and GFAP (red) immunofluorescence and Hoechst-stained nuclei (blue) in mouse BMV. f Collagen IV immunofluorescence of human BMV. g GFAP immunofluorescence of human BMV. h A composite image of of collagen IV (green) and GFAP (red) immunofluorescence and f Hoechst-stained nuclei (blue) in human BMV. i Hoechst staining of nuclei in human BMV. The scale bar is 20 µm