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. 2020 Jul 22;17:47. doi: 10.1186/s12987-020-00209-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The enrichment of the blood–brain barrier (BBB)-related proteins in mouse brain microvessels (BMV) compared to brain parenchyma. BMVs and vessel-free brain parenchyma were isolated as described in Materials and Methods and relative enrichment of key protein markers in each fractions was determined by proteomics. Shown are Log2 ratios between levels of various proteins in purified vessels and vessel-depleted parenchyma. Protein markers correspond to: (1) Endothelial cell markers: Slc2a1, Glucose transporter (Glut1); F8, Coagulation factor VIII-related antigen; Sele, E-selectin; Cdh5, VE-cadherin; Pecam1, CD31; (2) Pericyte markers: Pdgfrb, Platelet-derived growth factor receptor beta; Des, Desmin; Acta2, smooth muscle actin; Anpep: CD13 pericyte marker (Aminopeptidase N); (3) Astrocyte markers: Gfap, Glial fibrillary acidic protein; S100β, Protein S100-beta; Slc4a4, Electrogenic sodium bicarbonate cotransporter 1; Gja1, Gap junction alpha-1 protein; Aqp4, Aquaporin-4; (4) Structural proteins: Actb, Cytoplasmic actin; Tubb1, beta tubulin. Error bars represent mean ± SD of three biological replicates