Fig. 5.

TfR protein expression in isolated brain microvessels (BMVs). a Wes™ band density of TfR expression in human (h) and mouse (m) BMVs. b Quantification of TfR expression by Wes™ TfR in each lane is normalized to β-actin in the same lane; the fold-change of human TfR is relative to mouse TfR (set as one). Bars are mean ± SD of three biological replicates. The level of mTfR is significantly higher than hTfR (p < 0.05; unpaired two-tailed t-test). c–f Collagen IV (green) and TfR (red) immunofluorescence in human BMV. g–j CD31 (green) and TfR (red) immunofluorescence in mouse BMV. Nuclei are stained with Hoechst (blue) (f and j). k Negative controls for G–J. The scale bar is 20 µm