Table 2.
Expression levels of mouse genes encoding RMT receptors in isolated brain microvessels (BMV), whole brain, liver, spleen and lung vessels (RNAseq normalized read counts; average ± SD)
| Receptors/proteins | BMV | Brain | Liver | Spleen | Lung vessels |
|---|---|---|---|---|---|
| TFRC | 3690.57 ± 1577.32* | 1366.54 ± 198.69 | 824.73 ± 70.64 | 3112.77 ± 1853.22 | 358.11 ± 79.98 |
| INSR | 3577.43 ± 889.85* | 956.02 ± 215.79 | 1219.83 ± 877.37 | 561.32 ± 212.32 | 1475.28 ± 84.75 |
| IGF1R | 6876.68 ± 1430.93* | 1102.77 ± 297.56 | 38.19 ± 27.33 | 386.79 ± 70.17 | 1920.06 ± 367.45 |
| IGF2R | 952.45 ± 129.07 | 705.28 ± 248.38 | 413.13 ± 188.02 | 926.11 ± 358.19 | 1531.46 ± 133.94* |
| LRP1 | 11,506.40 ± 833.68* | 8489.28 ± 1692.30 | 4448.88 ± 920.24 | 3403.74 ± 934.22 | 6397.87 ± 829.14 |
| LDLR | 1015.42 ± 223.35 | 584.67 ± 170.78 | 2210.82 ± 1160.86 | 850.94 ± 230.47 | 1062.21 ± 116.65 |
| LRP8 (ApoER2) | 3937.6 ± 777.50* | 1318.88 ± 271.37 | 15.31 ± 20.31 | 310.13 ± 107.61 | 36.57 ± 12.07 |
| CDC50A/TMEM30A | 4159.55 ± 119.32* | 8495.71 ± 1376.15 | 13,191.59 ± 2020.86 | 2457.55 ± 424.88 | 3534.28 ± 715.38 |
| SLC2A1/GLUT1 | 33,418.54 ± 13,876.59*# | 2127.85 ± 246.70 | 216.14 ± 34.86 | 1164.86 ± 192.17 | 478.96 ± 48.42 |
| SLC3A2/CD98hc | 6369.22 ± 1046.86* | 2665.44 ± 27.16 | 979.66 ± 147.88 | 7792.42 ± 1947.03 | 5596.12 ± 929.74 |
| LEPR/leptin receptor | 356.37 ± 14.03* | 90.68 ± 1.77 | 107.97 ± 9.58 | 667.06 ± 116.16 | 1917.86 ± 469.42 |
Comparisons of gene expression (transcript abundance) across isolated mouse BMVs and peripheral tissues was performed using one-way ANOVA, followed by Tukey’s multiple comparisons test. For simplicity, statistical significance was shown only for comparisons of BMVs against other tissues (but not among peripheral tissues)
*TFRC expression in BMV was significantly (p < 0.05) higher compared to lung vessels
*INSR expression in BMV was significantly higher (p < 0.01) compared to brain, liver, lung vessels or spleen
*IGF1R expression in BMV was significantly (p < 0.0001) higher compared to brain, liver, spleen or lung vessels
*IGF2R expression in lung vessels was significantly higher compared to brain tissue (p < 0.01) and liver (p < 0.001)
*LRP1 expression in BMV was significantly (p < 0.01) higher compared to liver, spleen, or lung vessels
*LRP8 expression in BMV was significantly (p < 0.0001) higher compared to brain, liver, spleen or lung vessels
*CDC50A/TMEM30A expression in BMV was significantly (p < 0.01) lower compared to brain or liver
*SLC2A1/GLUT1 expression in brain vessels was significantly (p < 0.001) higher compared to brain tissue, liver, spleen and lung vessels
#SLC2A1/GLUT1 expression was significantly (p < 0.001) higher in BMV compared to the expression off all other genes shown in Table 2
*SLC3A2/CD98hc expression in BMV was significantly higher (p < 0.01) compared to brain or liver
*LEPR expression in BMV was significantly (p < 0.0001) lower compared to lung vessels or spleen and significantly (p < 0.001) higher compared to brain or liver