Table 3.
Expression levels of genes encoding RMT receptors in isolated human and mouse brain microvessels (BMV) and whole brain tissues [RNAseq normalized for transcript per million (TPM); Mean ± SD]
| Receptors/proteins | Human BMVs | Human brain | Mouse BMV | Mouse brain |
|---|---|---|---|---|
| TFRC | 8.81 ± 4.43 | 11.25 ± 2.80 | 70.53 ± 32.21* | 20.85 ± 4.41# |
| INSR | 5.44 ± 3.70 | 4.75 ± 0.49 | 33.09 ± 8.63* | 8.08 ± 3.31 |
| IGF1R | 2.38 ± 1.65 | 2.86 ± 0.69 | 61.06 ± 13.70* | 7.36 ± 2.33# |
| IGF2R | 3.09 ± 3.42 | 3.88 ± 2.06 | 9.88 ± 1.40* | 5.86 ± 2.57 |
| LRP1 | 21.18 ± 16.53 | 28.80 ± 6.61 | 179.85 ± 15.66* | 69.87 ± 15.36# |
| LDLR | 14.04 ± 19.25 | 5.19 ± 1.55 | 21.21 ± 5.00 | 9.67 ± 3.66 |
| LRP8 (ApoER2) | 6.09 ± 6.13 | 5.58 ± 1.75 | 20.66 ± 5.65 | 89.11 ± 20.41# |
| CDC50A/TMEM30A | 28.62 ± 16.30 | 68.42 ± 15.01 | 98.36 ± 1.58* | 158.57 ± 13.88# |
| SLC2A1/GLUT1 | 86.20 ± 88.50 | 41.64 ± 12.54 | 1314.53 ± 559.01* | 64.62 ± 1.08# |
| SLC3A2/CD98hc | 86.68 ± 75.46 | 89.37 ± 3.74 | 406.81 ± 66.31* | 138.96 ± 14.72# |
| LEPR/leptin receptor | 2.25 ± 1.96 | 1.19 ± 0.32 | 1.35 ± 1.35 | 2.10 ± 0.71 |
| GAPDH | 1563.77 ± 301.52 | 3924.81 ± 295.78 | 1571.31 ± 237.26 | 2824.45 ± 442.70& |
| S100B | 520.09 ± 180.37 | 1005.79 ± 308.03 | 547.96 ± 5.82 | 231.91 ± 33.28& |
| TUBB4A | 328.93 ± 126.15 | 363.04 ± 92.13 | 357.16 ± 110.37 | 552.25 ± 194.57 |
Statistical comparison of gene expression (transcript abundance) between human and mouse BMVs and human and mouse brain was performed using two-tailed student t-test. Significant difference between human and mouse BMVs was indicated by (*) and significant difference between human and mouse brain is indicated by (#), *Receptor abundance is significantly higher (TFRC p < 0.01; INSR p < 0.001; IGF1R p < 0.01; IGF2R p < 0.05; LRP1 p < 0.001; LRP8 p < 0.001; CDC50A p < 0.01; SLC3A2 p < 0.01; SLC2A1 p < 0.01) in mouse compared to human BMVs
#Receptor expression is significantly higher (TFRC p < 0.05; IGF1R p < 0.05; LRP1 p < 0.05; LRP8 p < 0.005; CDC50A p < 0.002; SLC2A1 p < 0.05; SLC3A2 p < 0.01) in mouse compared to human brain
&Genes encoding structural proteins: S100B is significantly (p < 0.001) lower in mouse compared to human brain; GAPDH is significantly lower in mouse compared to human brain (p < 0.05)