Table 2.
Functional annotation of hypothalamic DEGs in response to a variable growth rate of cockerels after 3rd and 6th weeks from hatching that was performed based on STRING and PANTHER tools
| Gene ontology | FDR | No. | 3-week-old | 6-week-old |
|---|---|---|---|---|
| GO Biological Process COMPLETE | ||||
| GO:0098883 synapse pruning | 1.35E-02 | C1QC, C1QB, C1QA | ||
| GO:0045080 positive regulation of chemokine biosynthetic process | 2.01E-02 | CD74, MYOD88, EGR1 | ||
| GO:0042403 thyroid hormone metabolic process | 3.27E-02 | 3 | CRYM, CGA, CPQ | |
| GO:0048513 organ development | 0.00216 | 10 | BSX, MGP, MMP2, MYH11, EGR1, PCDH15, FST, LECT2, TBX3, NPPC | |
| GO:0010469 regulation of signaling receptor activity | 2.26E-02 | 7 | GHRH, PMCH, CGA, NMU, POMC, OGN, NPPC | |
| GO:0030154 cell differentiation | 0.0424 | 9 | MGP, MMP2, MYH11, EGR1, PCDH15, FST, LECT2, NPPC, TAGLN | |
| GO:0009914 hormone transport |
2.10E-03 3.88E-03 |
5 7 |
GHRH, CRYM, CGA, SLCO1C1, TBX3 | SLCO1C1, CGA, TBX3, GAL, SLC30A8, BTK, TTR |
| GO:0010817 regulation of hormone levels | 2.32E-04 4.95E-02 |
16 13 |
CRYM, TBX3, GHRH, FGB, SLCO1C1, GGA, BCO1, ALDH6, TCF7L2,CGA, CPLX1, EGR1, CPQ, TRH, CHGA, POMC | TBX3, NPY, GAL, DIO2, SLC30A8, SLCO1C1, BLK, TCF7L2, CGA, TTR, NR5A1, EGR1, LHCGR, POMC |
| GO:0045187 regulation of circadian sleep/wake cycle | 0.0193 | 3 | PER3, KCNA2, GHR | |
| GO:0002052 positive regulation of neuroblast proliferation | 0.0178 | 3 | OTP, DCT, BF1 | |
| GO:0034341 response to interferon-gamma | 6.19E-04 | 7 | IRF9, CCL19, IFITM1, GBP1, IRF8, IRF7, IRF1 | |
| GO:0060337 type I interferon signaling pathway | 2.88E-02 | 3 | STAT2, STAT1, IRF3 | |
| GO molecular function complete | ||||
| GO:0071855 neuropeptide receptor binding | 5.77E-03 | 5 | GHRH, NPY, NMU, PMCH, POMC | |
| GO:0004029 aldehyde dehydrogenase (NAD) activity | 2.43E-02 | 2 | ALDH1A1, ALDH6 | |
| GO:001982 oxygen binding | 4.77E-02 | 2 | ALB, HBAD | |
| GO:0005179 hormone activity | 1.21E-05 | 11 | OXT1, GHRH, ENSGALG00000043381, RFLB, CNP3, NPY, CGA, TRH, AVP, PMCH, NMU, POMC, | OXT1, CNP3, NPY, POMC, CGA, GAL, PMCH, TTR, PNOC, KL, CALCA, TTR, CCK, |
| GO cellular component complete | ||||
| GO:0071855 neuropeptide receptor binding | 5.77E-03 | 5 | GHRH, NPY, NMU, PMCH, POMC | |
| GO:0004029 aldehyde dehydrogenase (NAD) activity | 2.43E-02 | 2 | ALDH1A1, ALDH6 | |
| GO:001982 oxygen binding | 4.77E-02 | 2 | ALB, HBAD | |
| GO:0005179 hormone activity | 1.21E-05 | 11 | OXT1, GHRH, ENSGALG00000043381, RFLB, CNP3, NPY, CGA, TRH, AVP, PMCH, NMU, POMC, | OXT1, CNP3, NPY, POMC, CGA, GAL, PMCH, TTR, PNOC, KL, CALCA, TTR, CCK, |
| GO cellular component complete | ||||
| GO:0062023 collagen-containing extracellular matrix | 1.63E-03 | 10 | COCH, MMP2, SPARCL1, COL28A1, MGP, COL6A2, COL4A6, COL1A2, COL1A1, EGF | |
| GO:0001664 G protein-coupled receptor binding | 2.10E-02 | 13 | PNOC, CCLI10, OXT1, GAL, CCL4, PDYN, CCLI7, NPY, RSPO3, PMCH, POMC, CCL19, ENSGALG00000003309 | |
| PANTHER GO-Slim Biological Process | ||||
| GO:0050796 regulation of insulin secretion | 3.85E-03 | 3 | CHGA, TCF7L2, TRH | |
| GO:0023056 positive regulation of signaling | 4.87E-03 | 6 | GHRH, TCF7L2, TRH | |
| GO:0043588 skin development | DSP, COL1A1, ENSGALG00000029182 | |||
| GO:0001501 skeletal system development | 1.35E-03 | 5 | COCH, COL6A2, COL6A1, COL1A1, ENSGALG00000029182 | |
| GO:0007631 feeding behavior | 0.03E-01 | 5 | BSX, PMCH, NPW, NMU, NKX2–1, | GAL, FOS, PMCH, BSX, STRA6 |
| GO:0006954 inflammatory response | 1.29E-02 | 8 | CCLI10, CCL4,CCR2, CCLI7, TLR1B, TLR2A, TLR3, CCL19 | |
| PANTHER Pathway | ||||
| T cell activation | 4.02E-04 | 8 | RAC2, DMB2, CD3E, B2M, CD3D, LCK, FOS, PTPRC, CD74, FOS, SLP76, CD3D | |
| B cell activation | 1.15E-03 | 7 | BLK, RAC2, BLNK, BTK, PTPN6, FOS, PTPRC | |
| 5-Hydroxytryptamine degredation | 8.40E-03 | 2 | ALDH1A1, ALDH6 | |
| Integrin signalling pathway | 1.32E-02 | 7 | COL1A2, COL4A6, COL3A1, COL6A1, COL6A2, ACTA2, COL1A1 | |
| Reactome pathways | ||||
| R-GGA-1650814.1 Collagen biosynthesis and modifying enzymes | 2.25E-03 | 5 | COL1A2, COL4A6, COL6A1, COL28A1, COL6A2 | |
| R-GGA-983170.1 Antigen Presentation: Folding, assembly and peptide loading of class I MHC | 2.35E-03 | 5 | ERAP1, BFIV21, B2M, BF1, TAP2 | |
| Integrin cell surface interactions | 6.70E-03 | 6 | FGB, COL6A2, COL4A6, ENSGALG00000026836, ENSGALG00000004946, COL6A1 | |
| R-GGA-977606.1 Regulation of Complement cascade | 6.21E-05 | 8 | SERPING1, ENSGALG00000030038, C1QC, C1R, CFH, C1S, C1QB, C1QA | |
| R-GGA-5686938.1 Regulation of TLR by endogenous ligand | 2.58E-02 | 4 | LY96, BPI, TLR1B, TLR2A | |