Fig. 4.

Ddx56 interacts with Oct4 and Sox2. a Ddx56-GST and Oct4-SFB plasmids were cotransfected into 293T cells, followed by IP and western blotting experiments. GST beads were used for pulldown. Negative controls: Ddx56-GST+GFP-SFB and GFP-GST+Oct4-SFB. Positive control: Sox2-GST+Oct4-SFB. b Ddx56-GST and Sox2-SFB plasmids were cotransfected into 293T cells, followed by IP and western blotting experiments. GST beads were used for pulldown. Negative controls: Ddx56-GST+GFP-SFB and GFP-GST+Sox2-SFB. Positive control: Oct4-GST+Sox2-SFB. c, d GST-tagged Ddx56 truncations and Sox2-HA plasmids were cotransfected into 293T cells, followed by IP and western blotting. e GST-tagged Ddx56 and Ddx56 ΔC-ter plasmids were transfected into 293T cells separately and measured by western blot. His-Sox2 protein was purified by Ni-sepharose purification. Using the same quantity of GST-Ddx56 and Ddx56 ΔC-ter separately, pulldown was performed to detect interaction