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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Stress. 2020 Mar 4;23(4):457–465. doi: 10.1080/10253890.2020.1733962

Figure 6.

Figure 6.

Levels of corticotropin releasing hormone (CRH) mRNA at baseline or after 20 minutes of restraint stress within the paraventricular nucleus (PVN) of pair- versus single-housed male mice exposed to chronic variable stress (CVS) or control conditions. Arbitrary density units (ADUs) calculated from in situ hybridization autoradiograms of CRH mRNA are shown in panel a. Each bar represents mean ± SEM, and numbers within each bar indicate the number of animals used for each group. *=p<0.05 and **=p<0.01 for restraint stressed males versus non-stressed males of the same treatment and housing condition. Panel b shows representative autoradiograms of CRH hybridization in the PVN of pair- and single-housed male mice. 3V= third ventricle.