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. 2020 May 28;20(2):1441–1451. doi: 10.3892/ol.2020.11683

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Copyright: © Jian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — AGR3 and ER are negatively regulated by 4-OH Tam in T47D cells. (A) The viability of T47D cells treated with 0–10 µmol 4-OH Tam was detected using the Cell Counting Kit-8 assay. (B) The protein levels of AGR3 and ER in T47D cells treated with 0, 2, 4, 6, 8 and 10 µmol 4-OH Tam for 24 h were detected by western blotting. (C and D) RNA expression levels of (C) ESR1 and (D) AGR3 were detected in T47D cells after the addition of different concentrations of 4-OH Tam for 24 h. The RNA expression level of 0 µmol was normalized to 1 in (C) and (D). Each experiment was repeated ≥3 times. **P<0.01 and ****P<0.0001 vs. 0 µmol. 4-OH Tam, 4-hydroxytamoxifen; AGR3, anterior gradient 3; ER, estrogen receptor; ESR1, estrogen receptor 1; ns, not significant.