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. 2020 May 28;20(2):1441–1451. doi: 10.3892/ol.2020.11683

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Copyright: © Jian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — AGR3 promotes T47D cell proliferation in an estrogen-dependent manner. (A) RNA levels of AGR3 were detected by RT-qPCR after knocking down AGR3 in T47D cells. (B) Protein levels of AGR3 and ER were detected by western blotting after knocking down AGR3 in T47D cells. (C) Cell viability was detected using the Cell Counting Kit-8 assay after knocking down AGR3 in T47D cells for 2 consecutive days. (D) RNA levels of AGR3 and ESR1 were detected by RT-qPCR after knocking down ESR1 in T47D cells. Each experiment was repeated ≥3 times. **P<0.01; ***P<0.001; ****P<0.0001 vs. si-NC. RT-qPCR, reverse transcription-quantitative PCR; siRNA, small interfering RNA; si-NC, small interfering negative control; AGR3, anterior gradient 3; ER, estrogen receptor; ESR1, estrogen receptor 1.