| Reagent | Volume per sample |
|---|---|
| Nuclease-free Water | 33 μL |
| 10× DNase Buffer | 5 μL |
| 10× (1%) Pluronic F-68 | 5 μL |
| DNase I, RNase-free | 2 μL (20 units) |
| Sample (AAV) | 5 μL |
| Total Volume | 50 μL |
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