| Reagent | Vol. per reaction (μL) |
|---|---|
| TaqMan™ Universal PCR Mix (2×) | 26.25 |
| Forward Primer (3 μM) | 5.25 |
| Reverse Primer (3 μM) | 5.25 |
| Fluorescent Probe (2 μM) | 5.25 |
| Nuclease-free water | 5.25 |
| Test Sample or control | 5.25 |
| Total | 52.50 |
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