Table 1.

Example ddPCR Report

Sample ID	Titer #1	Titer#2	Titer #3	Average	Stnd Dev	Notes
Sample A TFF	2.52E+12	2.43E+12	2.14E+12	2.36E+12	1.99E+11
Sample A Final	2.45E+13	2.17E+13	2.03E+13	2.22E+13	2.16E+12
1E8 IE-5 (Plasmid DNase-I Control)	9.25E+07	8.85E+07	6.35E+07	8.15E+07	1.57E+07	Copies per reaction
1E8D IE 5 (Plasmid DNase-I Control)	6.00E+05	1.55E+06	1.50E+06	1.22E+06	5.35E+05	Copies per reaction
M/B (Mock Control)	3.70E+09	2.30E+09	2.40E+09	2.80E+09	7.81E+08
XXXX (Validation Control)	1.20E+13	1.08E+13	9.71E+12	1.08E+13	1.16E+12
NTC	2.40E+00	8.00E+00	3.60E+00	4.67E+00	2.95E+00	Copies per reaction

In-process samples (TFF) and final concentrates are routinely quantified in our labs, as well as crude lysates. The latter require less dilution. The validation control samples are reproducible to within 10–30% with very low background each run (<0.01%). Intra-assay reproducibility is within 10–30% among different operators