Figure 3.

PTEN was the target of miR-494 in RB cells. (A) Schematic representation of the binding sites of miR-494 with PTEN 3′UTR. (B) Relative luciferase activity was tested after overexpression of miR-494 in wild-type (WT) or mutated (MuT) Y79 cells. (C) Relative luciferase activity detected in SO-RB50 cells after silence miR-494 in WT or MuT. (D) Relative protein level of PTEN by western blotting in Y79 cells or SO-RB50 cells. (E) Relative PTEN mRNA expression in Y79 cells or SO-RB50 cells by qRT-PCR. (F) The association of miR-494 expression and PTEN expression was measured by Spearman's Rank correlation. **P<0.01. RB, retinoblastoma; PTEN, phosphatase and tensin homolog.