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Treatment with IFN-γ induces a significant increase in PD-L1 protein expression levels in colorectal cancer cells. HCT 116 cells were treated with 10 or 20 ng/ml IFN-γ for 24 h. (A) Western blotting was used to determine the relative protein expression levels of PD-L1, JAK2, p-JAK2, STAT1 and p-STAT1. (B) Relative protein expression of PD-L1, p-JAK2/JAK2, p-STAT1/STAT1 following IFN-γ treatment. IFN, interferon; PD-L1, programmed death ligand 1; JAK, janus kinase; p-, phosphorylated.
