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. 2020 Jun 5;20(2):1989–1998. doi: 10.3892/ol.2020.11699

Figure 4.

Figure 4.

STAT3 inhibition sensitizes human diffuse intrinsic pontine glioma cells to radiation by interfering with DNA damage repair. SF8628 cells were treated with control vehicle (DMSO) or AG490. SF8628 cells were transfected with shCtrl or shSTAT3. (A and B) CCK-8 assays of control cells and STAT3-inhibited cells at 0 and 24 h after radiation treatment. Cell viability was analyzed using a CCK-8 assay, and absorbance was measured at 420 nm. *P<0.05 using one-way ANOVA. Data are presented as the mean ± SD. (C and D) Analysis of DNA damage repair after treatment with 4 Gy radiation by visualizing the double-strand marker γH2AX. The panel shows representative images of γH2AX (green), DAPI-stained nuclei (blue) and merged images of SF8628 cells without irradiation and at 1, 4 and 24 h after radiation. Scale bar, 1 µm. STAT3, signal transducer and activator of transcription 3; shCtrl, control short hairpin RNA; shSTAT3, STAT3 short hairpin RNA; CCK-8, Cell Counting Kit-8; γH2AX, phosphorylated H2A X variant histone; DAPI, 4′6′-diamidio-2-phenoylindole.