Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 23;128(7):077008. doi: 10.1289/EHP7030

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted.

PMC Copyright notice

Figure 3A is a set of two heat maps titled i P S C-C M Tox [90 minutes] and i P S C-C M Phys [90 minutes], plotting P C B 3, 2 prime-O H-P C B 3, 3 prime-O H-P C B 3, 4 prime-O H-P C B 3, 2 prime, 5 prime-d i O H-P C B 3, 2 prime-P C B 3 sulfate, 3-P C B 3 sulfate, 3 prime-P C B 3 sulfate, 4 prime-P C B 3 sulfate, 4 prime-M e O-P C B 3, 4-C I-B Q, P C B 11, 4-O H-P C B 11, 5-O H-P C B 11, 4-P C B 11 sulfate, 4-M e O-P C B 11, P C B 52, 4-O H-P C B 52, 4-P C B 52 sulfate, 4-M e O-P C B 52, P C B 95, P C B 126, (plus)-P C B 136, (minus)-P C B 136, and P C B 153 (y-axis) across micromolar, ranging from 0 to 0.01 in increments of 0.009, 0.01 to 0.1 in increments of 0.09, 0.1 to 1 in increments of 0.9, 1 to 10 in increments of 9, and 10 to 100 in increments of 90 (x-axis) for vehicle-normalized responses in percentage, ranging from 0 to 200 in increments of 50. Figure 3B is a set of two heatmaps titled i P S C-C M Tox [24 hours] and i P S C-C M Phys [24 hours], plotting P C B 3, 2 prime-O H-P C B 3, 3 prime-O H-P C B 3, 4 prime-O H-P C B 3, 2 prime, 5 prime-d i O H-P C B 3, 2 prime-P C B 3 sulfate, 3-P C B 3 sulfate, 3 prime-P C B 3 sulfate, 4 prime-P C B 3 sulfate, 4 prime-M e O-P C B 3, 4-C I-B Q, P C B 11, 4-O H-P C B 11, 5-O H-P C B 11, 4-P C B 11 sulfate, 4-M e O-P C B 11, P C B 52, 4-O H-P C B 52, 4-P C B 52 sulfate, 4-M e O-P C B 52, P C B 95, P C B 126, (plus)-P C B 136, (minus)-P C B 136, and P C B 153 (y-axis) across micromolar, ranging from 0 to 0.01 in increments of 0.009, 0.01 to 0.1 in increments of 0.09, 0.1 to 1 in increments of 0.9, 1 to 10 in increments of 9, and 10 to 100 in increments of 90 (x-axis) for vehicle-normalized responses in percentage, ranging from 0 to 200 in increments of 50. Figure 3C is a set of two heat maps titled i P S C-E C Tox and i P S C-E C Tubulogenesis, plotting P C B 3, 2 prime-O H-P C B 3, 3 prime-O H-P C B 3, 4 prime-O H-P C B 3, 2 prime, 5 prime-d i O H-P C B 3, 2 prime-P C B 3 sulfate, 3-P C B 3 sulfate, 3 prime-P C B 3 sulfate, 4 prime-P C B 3 sulfate, 4 prime-M e O-P C B 3, 4-C I-B Q, P C B 11, 4-O H-P C B 11, 5-O H-P C B 11, 4-P C B 11 sulfate, 4-M e O-P C B 11, P C B 52, 4-O H-P C B 52, 4-P C B 52 sulfate, 4-M e O-P C B 52, P C B 95, P C B 126, (plus)-P C B 136, (minus)-P C B 136, and P C B 153 (y-axis) across micromolar, ranging from 0 to 0.01 in increments of 0.009, 0.01 to 0.1 in increments of 0.09, 0.1 to 1 in increments of 0.9, 1 to 10 in increments of 9, and 10 to 100 in increments of 90 (x-axis) for vehicle-normalized responses in percentage, ranging from 0 to 200 in increments of 50. Figure 3D is a set of two heat maps titled H U V E C Tox and H U V E C Tubulogenesis, plotting P C B 3, 2 prime-O H-P C B 3, 3 prime-O H-P C B 3, 4 prime-O H-P C B 3, 2 prime, 5 prime-d i O H-P C B 3, 2 prime-P C B 3 sulfate, 3-P C B 3 sulfate, 3 prime-P C B 3 sulfate, 4 prime-P C B 3 sulfate, 4 prime-M e O-P C B 3, 4-C I-B Q, P C B 11, 4-O H-P C B 11, 5-O H-P C B 11, 4-P C B 11 sulfate, 4-M e O-P C B 11, P C B 52, 4-O H-P C B 52, 4-P C B 52 sulfate, 4-M e O-P C B 52, P C B 95, P C B 126, (plus)-P C B 136, (minus)-P C B 136, and P C B 153 (y-axis) across micromolar, ranging from 0 to 0.01 in increments of 0.009, 0.01 to 0.1 in increments of 0.09, 0.1 to 1 in increments of 0.9, 1 to 10 in increments of 9, and 10 to 100 in increments of 90 (x-axis) for vehicle-normalized responses in percentage, ranging from 0 to 200 in increments of 50. — Heatmaps of concentration-related effects of PCBs and their metabolites in different cell types and time points. (A) Effects in iPSC-CMs at 90 min after exposure (“Tox” is cell viability and “Phys” is beats per minute phenotypes). (B) Effects in iPSC-CMs at 24 h after exposure (“Tox” is cell viability and “Phys” is beats per minute phenotypes). (C) Effects in iPSC-ECs (“Tox” is cell viability at 24 h, and “Tubulogenesis” is total tube length at 18 h phenotype). (D) Effects in HUVECs (“Tox” is cell viability at 24 h, and “Tubulogenesis” is total tube length at 18 h phenotype). The effects at each concentration were normalized to respective vehicle controls (i.e., 100%, white color). Relative increases and decreases in the respective assay signal are shown as red and blue gradients. Chemical names and abbreviations of PCBs and metabolites are listed in Table S1. Data for each cell and phenotype are available in the Supplemental Excel file, tabs iPSC-CMs; iPSC-ECs; HUVECs. Note: CMs, cardiomyocytes; ECs, endothelial cells; HUVECs, human umbilical vein endothelial cells; iPSC, induced pluripotent stem cell; iPSC-CMs, iPSC-derived cardiomyocytes; iPSC-ECs, iPSC-derived endothelial cells; PCBs, polychlorinated biphenyls; ToxPi, Toxicological Prioritization Index.